book_cover_img The Korean Society of Marine Life Science Journal of Marine Life Science eISSN 2508-7134
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Year of Launching : 2016
Frequency : Twice a year (June 15, December 15)
Doi Prefix : 10.23005/ksmls.

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ISSN : 2508-7134(Online)
Journal of Marine Life Science Vol.1 No.1 pp.70-78
DOI :

Current Advances in Cryopreservation of Microalgae

Wahyu Sri Kunto Nugroho1, Do-A Kim1, Dong-Woo Kim1, Bon-Won Koo1, Young Baek Hur2, Hak Jun Kim1*
1Department of Chemistry, Pukyong National University, Busan 48513, Korea
2Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Namhae 52440, Korea

Corresponding Author Hak Jun Kim Department of Chemistry, Pukyong National University, Busan 48513, Korea E-mail : kimhj@pknu.ac.kr

Abstract

Microalgae are of significant importance for future biotechnological applications. Many microalgae banks or laboratories attempt to maintain various microalgae for further research purposes. Cryopreservation has been preferred to reduce a labor-intensive and costly routine sub-culturing. Cryopreservation can also diminish the genetic drift risk. However, cryopreservation as a long term storage of microalgae method are still in developing progress because it cannot be generalized for all microalgae. Microalgae types, cryoprotectant agents (CPAs) types, freezing and thawing methods are the most important factors that should be considered for cryopreservation. In this short review the basic principles and the current advanced of microalgae cryopreservation methods are discussed with a suggested starting parameters for microalgae cryopreservation.

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