book_cover_img The Korean Society of Marine Life Science Journal of Marine Life Science eISSN 2508-7134
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ISSN : 2508-7134(Online)
Journal of Marine Life Science Vol.2 No.1 pp.1-6
DOI :

Studies on Cryopreservation of Sperm in Sevenband grouper, Epinephelus septemfasciatus and Kelp grouper, E. bruneus
능성어, Epinephelus septemfasciatus와 자바리, E. bruneus의 정자동결보존에 관한 연구

Hyo Gun Kim1, Soo Chel Kim2, Doo Yong Kim3, Jung Woo Kim4, Kang Hee Kho1*
김효근1, 김수철2, 김두용3, 김정우4, 고강희1*
1Department of Fisheries Science, Chonnam National University, Yeosu 59626, Korea
2Department of Biomedical and Electronic Engineering, Chonnam National University, Yeosu 59626, Korea
3Department of Fisheries Technology Research, Ocean and Fisheries Science Institute, Wando-Gun 59116, Korea
4Department of Anatomy, College of Medicine, Seonam University, Namwon 55724, Korea

1전남대학교 수산해양대학 양식생물학
2전남대학교 공학대학 바이오전자메디컬공학
3전라남도 해양수산과학원
4서남대학교 의과대학 해부학교실
Corresponding Author Kang Hee Kho Department of Fisheries Science, Chonnam National University, Yeosu 59626, Korea E-mail : kkh@chonnam.ac.kr

Abstract

The purpose of this research is to develop the simple freezing method for sperm cryopreservation in the Sevenband grouper and Kelp grouper. The motility and survival rate were checked to investigate the effects of diluent and cryoprotectant on sperm cryopreservation.In cryopreservation experiments, 300 mM glucose and 15% dimethyl sulfoxide (DMSO) were used as a diluent and a cryoprotectant, respectively. As a results of cryopreservation, the motility rate of Sevenband grouper was found to be more than 90%, and that of Kelp grouper was found to be more that 60% in sperm 5 months after cryopreservation. 본 연구의 목적은 능성어와 자바리의 정자동결을 위한 간편한 실험법개발이다. 희석제와 동해 방지제가 정자동결에 미치는 효과를 파악하고자 운동성성과 생존율을 조사하였다. 동결실험에 서 300 mM glucose와 15% dimethylsulfoxide (DMSO)를 희석제와 동해방지제로 각각 사용하였 다. 동결실험결과, 능성어 정자는 동결 5개월 후 60% 이상의 운동성을 보였고, 자바리 정자는 동결 5개월후 90% 이상의 운동성을 보였다.

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