The Korean Society of Marine Life Science
Journal of Marine Life Science eISSN 2508-7134
Year of Launching : 2016
Frequency : Twice a year (June 15, December 15)
Doi Prefix : 10.23005/ksmls.
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Characterization of L-asparaginase-producing Trichoderma spp. Isolated from Marine Environments
ISSN : 2508-7134(Online)
Journal of Marine Life Science Vol.7 No.2 pp.121-128
DOI : https://dx.doi.org/10.23005/ksmls.2022.7.2.121
DOI : https://dx.doi.org/10.23005/ksmls.2022.7.2.121
Characterization of L-asparaginase-producing Trichoderma spp. Isolated from Marine Environments
Abstract
L-asparaginase (ASNase) is a therapeutic enzyme used to treat acute lymphoblastic leukemia. Currently, the most widely used ASNases are originated from bacteria. However, owing to the adverse effects of bacterial ASNases, new resources for ASNase production should be explored. Fungal enzymes are considered efficient and compatible resources of natural products for diverse applications. In particular, fungal species belonging to the genus Trichoderma are well-known producers of several commercial enzymes including cellulase, chitinase, and xylanase. However, enzyme production by marine-derived Trichoderma spp. remains to be elucidated. While screening for extracellular ASNase-producing fungi from marine environments, we found four strains showing extracellular ASNase activity. Based on the morphological and phylogenetic analyses using sequences of translation elongation factor 1-alpha (tef1α), the Trichoderma isolates were identified as T. afroharzianum, T. asperellem, T. citrinoviride, and Trichoderma sp. 1. All four strains showed different ASNase activities depending on the carbon sources. T. asperellem MABIK FU00000795 showed the highest ASNase value with lactose as a carbon source. Based on our findings, we propose that marine-derived Trichoderma spp. are potential candidates for novel ASNase production.