The Korean Society of Marine Life Science
Journal of Marine Life Science eISSN 2508-7134
Year of Launching : 2016
Frequency : Twice a year (June 15, December 15)
Doi Prefix : 10.23005/ksmls.
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ISSN : (Print)
ISSN : 2508-7134(Online)
ISSN : 2508-7134(Online)
Journal of Marine Life Science Vol.8 No.2 pp.115-120
DOI : https://dx.doi.org/10.23005/ksmls.2023.8.2.115
DOI : https://dx.doi.org/10.23005/ksmls.2023.8.2.115
Sperm Cryopreservation of Korean Bullhead Pseudobagrus fulvidraco
September 27, 2023 ;
October 04, 2023 ;
October 23, 2023
Abstract
This study aims to find out a suitable extender and cryoprotective agent (CPA) for cryopreservation and its optimum concentration in order to conduct planned artificial seed production of Korean bullhead Pseudobagrus fulvidraco and to preserve superior sperm. Experiments were designed to investigate the effects of the different combinations of three extenders (Ⅰ: 300 mM glycose, Ⅱ: Kurokura extender, Ⅲ: Li extender), four cryoprotectants (dimethyl sulfoxide, ethylene glycol, methanol and glycerol) and four concentrations (5, 10, 15, 20%) on the cryopreservation of Korean bullhead sperm. Postthawed sperm survival rate and sperm activity index (SAI) were detected to evaluate the effects of sperm cryopreservation. The optimal combination of extender and CPA for cryopreservation of Korean bullhead sperm was extender Ⅲ + 10 and 15% methanol, resulting in a survival rate and SAI of 66.9 ± 8.7, 67.3 ± 13.1% and 2.6 ± 0.4, 2.6 ± 0.5 respectively, which was higher than had been achieved with other extenders and CPAs.
동자개 Pseudobagrus fulvidraco 정자 동결보존
초록
본 연구는 동자개 정자의 동결보존을 위해 동결보존제의 최적 농도와 적정 희석액을 구명하여 정자를 최상의 상태로 보존하여 인공종자를 생산하는데 목적이 있다. 실험은 3종의 희석액(Ⅰ: 300 mM glycose, Ⅱ: Kurokura extender, Ⅲ: Li extender), 4종의 동결보존제(dimethyl sulfoxide, ethylene glycol, methanol and glycerol)와 4개의 동결보존제 농도(5, 10, 15, 20%)를 조합하여 대하여 조사하였다. 동결보존한 정자를 해동한 후 정자의 생존율과 정자활성지수로 동결보존 제의 효과를 평가하였다. 희석액 Ⅲ(Li extender)과 10과 15%의 methanol을 조합했을 때 동자 개 정자의 생존율과 정자활성지수는 각각 66.9 ± 8.7, 67.3 ± 13.1%과 2.6 ± 0.4, 2.6 ± 0.5로 다른 희석액과 동결보존제보다 높았다.